Potent enhancement of transdermal absorption and stability of human tyrosinase plasmid (pAH7/Tyr) by Tat peptide and an entrapment in elastic cationic niosomes

Autor:	Jiradej Manosroi, Rolf G. Werner, Aranya Manosroi, Friedrich Götz, Narinthorn Khositsuntiwong, Worapaka Manosroi
Rok vydání:	2013
Předmět:	Male Materials science Skin Absorption Tyrosinase Molecular Sequence Data Pharmaceutical Science Administration Cutaneous Rats Sprague-Dawley chemistry.chemical_compound Plasmid Drug Stability Dermis Cations medicine Animals Humans Amino Acid Sequence Niosome Liposome Chromatography Ethanol Monophenol Monooxygenase Cationic polymerization General Medicine Elasticity Peptide Fragments Rats Up-Regulation medicine.anatomical_structure Transdermal absorption chemistry Gene Products tat Liposomes Plasmids
Zdroj:	Drug Delivery. 20:10-18
ISSN:	1521-0464 1071-7544
Popis:	Enhancement of transdermal absorption through rat skin and stability of the human tyrosinase plasmid (P) using Tat (T) and an entrapment in elastic cationic niosomes (E) were described. E (Tween61:cholesterol:DDAB at 1:1:0.5 molar ratio) were prepared by the freeze-dried empty liposomes (FDELs) method using 25% ethanol. TP was prepared by a simple mixing method. TPE was prepared by loading T and P in E at the T:P:E ratio of 0.5:1:160 w/w/w. For gel formulations, P, TP, PE and TPE were incorporated into Carbopol 980 gel (30 µg of plasmid per 1 g of gel). For the transdermal absorption studies, the highest cumulative amounts and fluxes of the plasmid in viable epidermis and dermis (VED) were observed from the TPE of 0.31 ± 0.04 µg/cm(2) and 1.86 ± 0.24 µg/cm(2)/h (TPE solution); and 4.29 ± 0.40 µg/cm(2) and 25.73 ± 2.40 µg/cm(2)/h (TPE gel), respectively. Only plasmid from the PE and TPE could be found in the receiving solution with the cumulative amounts and fluxes at 6 h of 0.07 ± 0.01 µg/cm(2) and 0.40 ± 0.08 µg/cm(2)/h (PE solution); 0.10 ± 0.01 µg/cm(2) and 0.60 ± 0.06 µg/cm(2)/h (TPE solution); 0.88 ± 0.03 µg/cm(2) and 5.30 ± 0.15 µg/cm(2)/h (PE gel); and 1.02 ± 0.05 µg/cm(2) and 6.13 ± 0.28 µg/cm(2)/h (TPE gel), respectively. In stability studies, the plasmid still remained at 4 ± 2 °C and 25 ± 2 °C of about 48.00-65.20% and 27.40-51.10% (solution); and 12.34-38.31% and 8.63-36.10% (gel), respectively, whereas at 45 ± 2 °C, almost all the plasmid was degraded. These studies indicated the high potential application of Tat and an entrapment in elastic cationic niosomes for the development of transdermal gene delivery system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5884a16c55cef5a573abec2b1be6981 https://doi.org/10.3109/10717544.2012.742937 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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