Increased coronary arteriolar contraction to serotonin in juvenile pigs with metabolic syndrome

Autor: Zhiqi Zhang, Vasile I Pavlov, Isabella Lawandy, Reed Jaworski, Yuhong Liu, Guangbin Shi, Frank W. Sellke, Jun Feng, Laura A. Scrimgeour
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Contraction (grammar)
Vascular smooth muscle
Swine
Indomethacin
Receptors
Thromboxane
Clinical Biochemistry
0302 clinical medicine
Medicine
Microvascular constriction
Receptor
Metabolic Syndrome
2. Zero hunger
biology
General Medicine
Receptor antagonist
Coronary Vessels
Arterioles
Hydrazines
Quinacrine
030220 oncology & carcinogenesis
Fatty Acids
Unsaturated
Pigs
Coronary arterioles
Serotonin
medicine.medical_specialty
Clinical chemistry
medicine.drug_class
Article
Juvenile obesity
Thromboxane A2
03 medical and health sciences
Phospholipase A2
Internal medicine
Animals
Molecular Biology
business.industry
Cell Biology
Bridged Bicyclo Compounds
Heterocyclic
medicine.disease
Disease Models
Animal
Phospholipases A2
030104 developmental biology
Endocrinology
Prostaglandin-Endoperoxide Synthases
Vasoconstriction
15-Hydroxy-11 alpha
9 alpha-(epoxymethano)prosta-5
13-dienoic Acid
biology.protein
Metabolic syndrome
business
Zdroj: Molecular and Cellular Biochemistry
ISSN: 1573-4919
0300-8177
DOI: 10.1007/s11010-019-03589-6
Popis: Metabolic syndrome (MetS) is associated with alterations in coronary vascular smooth muscle and endothelial function. The current study examined the contractile response of the isolated coronary arterioles to serotonin in pigs with and without MetS and investigated the signaling pathways responsible for serotonin-induced vasomotor tone. The MetS pigs (8-weeks old) were fed with a hyper-caloric, fat/cholesterol diet and the control animals (lean) were fed with a regular diet for 12 weeks (n = 6/group). The coronary arterioles (90–180 μm in diameter) were dissected from the harvested pig myocardial tissues and the in vitro coronary arteriolar response to serotonin was measured in the presence of pharmacological inhibitors. The protein expressions of phospholipase A2 (PLA2), TXA2 synthase, and the thromboxane-prostanoid (TP) receptor in the pigs’ left ventricular tissue samples were measured using Western blotting. Serotonin (10−9–10−5 M) induced dose-dependent contractions of coronary-resistant arterioles in both non-MetS control (lean) and MetS pigs. This effect was more pronounced in the MetS vessels compared with those of non-MetS controls (lean, P
Databáze: OpenAIRE
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