TXNIP Interaction with GLUT1 Depends on PI(4,5)P(2)
| Autor: | Cassi LaRose, Ning Wu, Holly Dykstra, Gongpu Zhao, Chelsea Fisk, Xing Meng, Althea N. Waldhart |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Phosphatidylinositol 4
5-Diphosphate endocrine system Glucose uptake Biophysics Endocytosis Biochemistry Article chemistry.chemical_compound Downregulation and upregulation Humans Phosphatidylinositol Glucose Transporter Type 1 biology Chemistry nutritional and metabolic diseases Isothermal titration calorimetry Biological Transport Cell Biology Lipids Clathrin Cell biology carbohydrates (lipids) Glucose biology.protein GLUT1 Carrier Proteins hormones hormone substitutes and hormone antagonists Intracellular TXNIP Signal Transduction |
| Zdroj: | Biochim Biophys Acta Biomembr |
| Popis: | GLUT1 is a major glucose facilitator expressed ubiquitously among tissues. Upregulation of its expression plays an important role in the development of many types of cancer and metabolic diseases. Thioredoxin-interacting protein (TXNIP) is an α-arrestin that acts as an adaptor for GLUT1 in clathrin-mediated endocytosis. It regulates cellular glucose uptake in response to both intracellular and extracellular signals via its control on GLUT1‐4. In order to understand the interaction between GLUT1 and TXNIP, we generated GLUT1 lipid nanodiscs and carried out isothermal titration calorimetry and single-particle electron microscopy experiments. We found that GLUT1 lipid nanodiscs and TXNIP interact in a 1:1 ratio and that this interaction requires phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 or PIP2). |
| Databáze: | OpenAIRE |
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