Massive accumulation of M and H subunits of neurofilament proteins in spinal motor neurons of neurofilament deficient Japanese quail, Quv
| Autor: | Michio Kobayashi, Masashiro Sugawara, Sumio Watanabe, Osamu Masamune, Itaru Toyoshima, Chizu Wada, Kazumaro Kato, Satoshi Okawa |
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| Rok vydání: | 2000 |
| Předmět: |
Neurofilament
Protein subunit Immunoblotting Central nervous system Chick Embryo Coturnix Axonal Transport Neurofilament Proteins biology.animal medicine Animals Motor Neuron Disease Phosphorylation Motor Neurons biology General Neuroscience Antibodies Monoclonal Anatomy Motor neuron Spinal cord Quail Cell biology Disease Models Animal medicine.anatomical_structure Axoplasmic transport Sciatic nerve |
| Zdroj: | Neuroscience Letters. 287:175-178 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(00)01148-4 |
| Popis: | Quiver (Quv) is a non-sense mutation of neurofilament protein L subunit (NF-L) that causes neurofilament deficiency with preserved microtubules in Japanese quail. Anti-NF-M and anti-NF-H mAbs stained cell bodies of motor neurons in Quv embryo spinal cords much more intense than those in control spinal cords. Volume of motor neurons in Quv spinal cords increased to 2.3 times of control motor neurons. Immunoblot of Quv spinal cords revealed a relative increase in non- and hypo-phosphorylated NF-M and NF-H, and a decrease in the total amount of NFs. Quv sciatic nerves showed faintly reacted phosphorylated NF-M and NF-H. These results suggest that deficiency of assembled neurofilament results in decreased axonal transport of NFs and accumulation of NFs in cell bodies of spinal motor neurons. |
| Databáze: | OpenAIRE |
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