Alteration of collapsin response mediator protein-2 expression in focal ischemic rat brain
| Autor: | Ji Eun Lee, Seung Taek Lee, Moon Jeong Ko, Hwa Jung Kim, Jeong Young Lee, Myung Ah Chung |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Nervous system Middle Cerebral Artery medicine.medical_specialty Immunoblotting Molecular Sequence Data Central nervous system Ischemia Tetrazolium Salts Nerve Tissue Proteins Brain Ischemia Rats Sprague-Dawley Brain ischemia Mediator Internal medicine Gene expression medicine Animals Electrophoresis Gel Two-Dimensional Amino Acid Sequence Coloring Agents business.industry General Neuroscience Collapsin response mediator protein-2 Infarction Middle Cerebral Artery Rat brain medicine.disease Rats Up-Regulation Carotid Arteries medicine.anatomical_structure Endocrinology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Intercellular Signaling Peptides and Proteins business |
| Zdroj: | NeuroReport. 16:1647-1653 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/01.wnr.0000176520.49841.e6 |
| Popis: | Collapsin response mediator protein-2 is highly expressed in the adult brain and it has been speculated to play roles in nervous system diseases. Studies determined for the first time whether collapsin response mediator protein-2 expression is altered in brain ischemia. We observed ischemia-modulated expression of different-sized collapsin response mediator protein-2. As the ischemic duration increased, the expression level of a known major 62 kDa collapsin response mediator protein-2 was decreased, whereas the expression of a newly-detected 58 kDa collapsin response mediator protein-2 was clearly increased in middle carotid artery-occluded rat brain tissues. Analysis of the two collapsin response mediator protein-2 bands revealed that the novel 58 kDa collapsin response mediator protein-2 observed in middle carotid artery-occluded rat brain tissues was a cleavage form; the predicted cleavage site is located at the carboxy-terminal of the collapsin response mediator protein-2. These data suggest that collapsin response mediator protein-2 is an important candidate controlling ischemic stroke. |
| Databáze: | OpenAIRE |
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