Interlaboratory Trial on the Determination of the In Vitro Iron Dialysability from Food
| Autor: | Richard F. Hurrell, Joop Luten, Kirsty Hickson, Peter Kastenmayer, Ulrich Schlemmer, Peter Van Dael, Lihua Shen, Helen M. Crews, Albert Flynn, Hendrik Deelstra, Susan J. Fairweather-Tait, Wenche Frøhlich, Rosaura Farré |
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| Rok vydání: | 1996 |
| Předmět: |
in vitro dialysability
Reproducibility Nutrition and Dietetics Chromatography biology Chemistry Pharmacology. Therapy interlaboratory trial Intestinal digestion Repeatability In vitro Gastrointestinal digestion Bioavailability iron Pepsin Multicenter study Rijksinstituut voor Visserijonderzoek biology.protein Netherlands Institute for Fisheries Research bioavailability Biology Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture, 72(4), 415-424 Journal of the Science of Food and Agriculture 72 (1996) 4 Journal of the science of food and agriculture |
| ISSN: | 1097-0010 0022-5142 |
| Popis: | An in vitro method for the estimation of iron bioavailability was subjected to an interlaboratory trial. The method involved a simulated gastrointestinal digestion using pepsin for the gastric stage followed by pancreatin and bile salts for the intestinal stage. The proportion of iron diffused through a semipermeable membrane (molecular mass cut-off 10 kDa) was used to measure the iron dialysability. An interlaboratory trial between nine laboratories was conducted to evaluate the repeatability and reproducibility of the agreed method. The reproducibility of the method among the participating laboratories was 20-30% and depended on the content of dialysable iron. Several factors contributing to the variation in the in vine dialysability among laboratories are discussed. The pH adjustment in the intestinal digestion was identified as one of the critical parameters. The present in vitro method was used to evaluate the iron dialysability from three meals. The dialysability data were in reasonable agreement with human absorption data. The usefulness of the in vitro dialysability method is discussed. |
| Databáze: | OpenAIRE |
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