Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains
| Autor: | Sophie Lafay, Philippe Langella, Virgile Guéneau, Angel Gil-Izquierdo, Pierre-Yves Mousset, Sonia Medina, Romain Briandet, Julien Deschamps, Luis G. Bermúdez-Humarán, Celia Chamignon |
|---|---|
| Přispěvatelé: | Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Indigo Therapeutics, Centro de Edafologia y Biologia aplicada del Segura (CEBAS - CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) [SDV]Life Sciences [q-bio] 030106 microbiology Microbiology Article biofilm law.invention 03 medical and health sciences Probiotic law Virology Lactobacillus medicine lcsh:QH301-705.5 ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Gastrointestinal tract Intestinal permeability biology stress tolerance Biofilm biology.organism_classification medicine.disease In vitro 3. Good health 030104 developmental biology Enzyme chemistry lcsh:Biology (General) probiotics permeability Bacteria |
| Zdroj: | Microorganisms Microorganisms, MDPI, 2020, 8 (7), pp.1053. ⟨10.3390/microorganisms8071053⟩ Volume 8 Issue 7 Microorganisms, Vol 8, Iss 1053, p 1053 (2020) |
| ISSN: | 2076-2607 |
| Popis: | Over the last 20 years, Lactobacillus species inhabiting the gastrointestinal tract (GIT) have received much attention, and their health-promoting properties are now well-described. Probiotic effects cannot be generalized, and their uses cover a wide range of applications. It is thus important to proceed to an accurate selection and evaluation of probiotic candidates. We evaluate the probiotic potential of six strains of Lactobacillus in different in vitro models representing critical factors of either survival, efficacy, or both. We characterized the strains for their ability to (i) modulate intestinal permeability using transepithelial electrical resistance (TEER), (ii) form biofilms and resist stressful conditions, and (iii) produce beneficial host and/or bacteria metabolites. Our data reveal the specificity of Lactobacillus strains to modulate intestinal permeability depending on the cell type. The six isolates were able to form spatially organized biofilms, and we provide evidence that the biofilm form is beneficial in a strongly acidic environment. Finally, we demonstrated the ability of the strains to produce &gamma aminobutyric acid (GABA) that is involved in the gut-brain axis and beneficial enzymes that promote the bacterial tolerance to bile salts. Overall, our study highlights the specific properties of Lactobacillus strains and their possible applications as biofilms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|