Identification of intracellular targets of small molecular weight chemical compounds using affinity chromatography
| Autor: | Stéphane Bach, Deborah Tribouillard, Laurent Meijer, Hervé Galons, Damien Guiffant, Marc Blondel, Fabienne Gug |
|---|---|
| Přispěvatelé: | Molécules et cibles thérapeutiques (MCT), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Drug
MESH: Microchemistry media_common.quotation_subject MESH: Pharmaceutical Preparations Biology MESH: Drug Design Applied Microbiology and Biotechnology Chromatography Affinity 03 medical and health sciences Biopolymers 0302 clinical medicine Affinity chromatography Molecule [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Protein kinase A 030304 developmental biology media_common chemistry.chemical_classification 0303 health sciences Microchemistry MESH: Molecular Weight General Medicine MESH: Chromatography Affinity 3. Good health Molecular Weight MESH: Biopolymers Enzyme Pharmaceutical Preparations chemistry Biochemistry Drug Design 030220 oncology & carcinogenesis MESH: Subcellular Fractions Molecular Medicine Selectivity Intracellular Subcellular Fractions |
| Zdroj: | Biotechnology Journal Biotechnology Journal, Wiley-VCH Verlag, 2007, 2 (1), pp.68-75. ⟨10.1002/biot.200600223⟩ Biotechnology Journal, 2007, 2 (1), pp.68-75. ⟨10.1002/biot.200600223⟩ |
| ISSN: | 1860-6768 1860-7314 |
| DOI: | 10.1002/biot.200600223⟩ |
| Popis: | Efforts to characterize small molecular weight chemical inhibitors of pharmacological interest tend to identify molecules with high efficiency and selectivity, to meet the two criteria required for the clinical development of a drug: efficacy and harmlessness. Drug candidates are expected to inhibit efficiently the target they have been optimized against (for example, a particular type of protein kinase). These hits are also designed to not interfere (or as little as possible) with the activity of other cellular enzymes/proteins to reduce undesired side effects. Here we discuss the use of immobilized drugs as affinity chromatography matrices to purify and identify their bona fide intracellular targets. This method not only allows the systematic investigation of the selectivity of pharmacological compounds but also the anticipation of their putative adverse effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text