Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements
| Autor: | Paolo Ugo, Emmanuele Ambrosi, Chiara Gaetani, Ligia Maria Moretto |
|---|---|
| Přispěvatelé: | Gaetani, Chiara, Ambrosi, Emmanuele, Ugo, Paolo, Moretto, LIGIA MARIA |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
food.ingredient electrochemical immunosensor Electrochemistry 01 natural sciences Horseradish peroxidase Analytical Chemistry lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound food Yolk Settore CHIM/01 - Chimica Analitica lcsh:QD415-436 immunoglobulin Y egg yolk nanoelectrodes ensembles food supplements SDS-PAGE Physical and Theoretical Chemistry nanoelectrodes ensemble High concentration Chromatography biology 010401 analytical chemistry 0104 chemical sciences 030104 developmental biology food supplement chemistry biology.protein Immunoglobulin Y Redox mediator Biosensor Methylene blue |
| Zdroj: | Chemosensors, Vol 5, Iss 1, p 10 (2017) Chemosensors; Volume 5; Issue 1; Pages: 10 |
| ISSN: | 2227-9040 |
| Popis: | Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labeled with horseradish peroxidase. In the presence of H2O2 and methylene blue as the redox mediator, an electrocatalytic current is generated which scales with IgY concentration in the sample. After optimizing the extracting procedure, the immunosensor was applied for analysis of fresh eggs and food integrators. The data obtained with the biosensor were validated by SDS-PAGE and Western blot measurements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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