Data on characterization and validation of assays for ultrasensitive quantitative detection of small molecules: Determination of free thyroxine with magnetic and interferometric methods
| Autor: | A. V. Orlov, S.L. Znoyko, A.V. Pushkarev, A.V. Lunin, E.N. Mochalova, N. V. Guteneva, Maxim P. Nikitin, Petr I. Nikitin |
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| Rok vydání: | 2018 |
| Předmět: |
medicine.drug_class
Nanoparticle 02 engineering and technology Magnetic particle inspection Monoclonal antibody lcsh:Computer applications to medicine. Medical informatics 01 natural sciences Dynamic light scattering medicine lcsh:Science (General) Multidisciplinary Chromatography Chemistry 010401 analytical chemistry 021001 nanoscience & nanotechnology Ligand (biochemistry) equipment and supplies Small molecule 0104 chemical sciences Dissociation constant Magnetic nanoparticles lcsh:R858-859.7 0210 nano-technology human activities lcsh:Q1-390 |
| Zdroj: | Data in Brief Data in Brief, Vol 21, Iss, Pp 1603-1611 (2018) |
| ISSN: | 2352-3409 |
| Popis: | The presented data refer to optimization and quantitative characterization of a rapid lateral flow assay based on high-affinity bifunctional ligand and magnetic nanolabels, which was developed for detection of small molecules of thyroid hormones. The results were obtained by several techniques, including the magnetic particle quantification method, spectral-correlation interferometry and spectral-phase interferometry, dynamic light scattering, enzyme linked immunosorbent assay. The long-term stability of “antibody – magnetic nanoparticle” conjugates is shown. The assay specificity is confirmed, and verification of successful combination of magnetic particles and antibodies is demonstrated. The kinetic and equilibrium dissociation constants are determined for interactions between thyroxine and monoclonal antibodies. The obtained data could be used for design of other platforms for detection of small molecules. |
| Databáze: | OpenAIRE |
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