Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?
| Autor: | Mehdi Hajian, Heydar Sadeghi, Mohsen Forouzanfar, S.M. Hosseini, F. Moulavi, Laleh Hosseini, Sh. Eghbalsaied, Hamid Bahramian, P. Abedi, Vajihe Asgari, Mohammad Hossein Nasr-Esfahani, M. Safahani Langrroodi, S. Ostadhosseini |
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| Rok vydání: | 2009 |
| Předmět: |
Animal Experimentation
Antioxidant medicine.medical_treatment Biology Cryopreservation Antioxidants Andrology Embryo Culture Techniques Freezing Genetics medicine Animals Vitrification Blastocyst Genetics (clinical) Mercaptoethanol Embryogenesis Obstetrics and Gynecology Embryo General Medicine Embryo Transfer Embryo transfer Culture Media medicine.anatomical_structure Reproductive Medicine Cell culture Cattle Female Developmental Biology |
| Zdroj: | Journal of assisted reproduction and genetics. 26(6) |
| ISSN: | 1573-7330 |
| Popis: | To determine the most optimal stage for antioxidant supplementation of culture medium to improve developmental competence, cryotolerance and DNA-fragmentation of bovine embryos.Presumptive zygotes were first cultured in presence or absence of beta-mercaptoethanol (beta-ME), for 8 days. Subsequently, half of the expanded blastocysts developed in both groups were vitrified, warmed within 30 min and post-warming embryos along with their corresponding non-vitrified embryos were cultured for two further days in presence or absence of (100 microM) betaME.For vitrified and non-vitrified embryos, the best effect was found when betaME was added from day 1 of in vitro culture in continuation with post-warming culture period. Day 1-8 supplementation significantly increased the rates of cleavage, day 7 and day 8 blastocyst production. For non-vitrified embryos, betaME addition during day 1-8 and/or 9-10 of embryo culture improved both hatching rate and quality of hatched embryos. For vitrified embryos, however, the percentage of DNA-fragmentation (18.5%) was significantly higher (por = 0.05) than that of embryos developed in absence of betaME but supplemented with betaME during post-warming period (13.5%).Exogenous antioxidant increases the chance of embryos, even those of fair-quality, to develop to blastocyst. However, antioxidant inclusion during in vitro embryo development is not sufficient to maintain the redox state of these embryos during the critical period of post-warming embryo culture, and therefore, there should be a surplus source of exogenous antioxidant during post-warming embryo culture. |
| Databáze: | OpenAIRE |
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