Comparison of aqueous and enzymatic extraction combination with sequential filtration for the profiling of selected trace elements in medicinal plants from Kenya
| Autor: | David K. Kariuki, M. Z. Getenga, Volker Nischwitz, Richard Mogwasi |
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| Rok vydání: | 2019 |
| Předmět: |
Chromium
010501 environmental sciences 01 natural sciences Biochemistry Aloe vera Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Nickel Plectranthus barbatus Medicinal plants 0105 earth and related environmental sciences Molybdenum chemistry.chemical_classification Manganese Plants Medicinal Aqueous solution biology Traditional medicine Chemistry Water Vanadium Cobalt biology.organism_classification Kenya Trace Elements Bioavailability Zinc Enzyme Bidens pilosa Molecular Medicine Solanum mauense Copper Filtration 030217 neurology & neurosurgery |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 54:1-7 |
| ISSN: | 0946-672X |
| Popis: | This work presents results for the profiling of eight essential elements (Co, Cu, Ni, V, Mo, Mn, Zn and Cr) in aqueous and enzymatic extracts of eight anti-diabetic medicinal plants, used by Traditional Medicine Practitioners from Nyamira County, Kenya determined by ICP-MS. The plants used in the study were Solanum indicum, Plectranthus barbatus, Ultrica dioica, Bidens pilosa, Solanum mauense, Clerodendrum myricoides, Carissa edulis and Aloe vera. A sequential filtration procedure was applied to fractionate the elemental contents of the obtained aqueous extracts into molecular size fractions. The results indicate that the low molecular size species (3 kDa) were predominant for Mo, Zn, Ni, Co, Mn and Cu, while the moderately large species (10 kDa-0.45 μm) of V were predominant in most of the medicinal plant extracts. In addition enzymatic extraction was compared to aqueous extraction to study the effect of the gastric and intestinal conditions on the release of selected elements from the plants. The amount of the elements extracted by the gastric phase enzymes was higher than the amount extracted by the intestinal phase enzymes. In general, the determined elemental amounts of enzymatic extractions were higher than those of corresponding water extractions for 70% of the elements studied. |
| Databáze: | OpenAIRE |
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