Impaired Tight Junctions in Atopic Dermatitis Skin and in a Skin-Equivalent Model Treated with Interleukin-17
| Autor: | Megumi Tobiishi, Yoshiki Tokura, Yukiko Ota, Takuo Yuki, Ayumi Kusaka-Kikushima |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Keratinocytes Pathology Physiology Stratum granulosum Cell Membranes lcsh:Medicine Filaggrin Proteins Pathology and Laboratory Medicine Epithelium 0302 clinical medicine Animal Cells Immune Physiology Claudin-1 Medicine and Health Sciences lcsh:Science Barrier function Cells Cultured Skin Innate Immune System Multidisciplinary Tight junction integumentary system Interleukin-17 Interleukin Atopic dermatitis medicine.anatomical_structure 030220 oncology & carcinogenesis Physical Sciences Amino Acid Analysis Cytokines Anatomy Integumentary System Junctional Complexes Cellular Structures and Organelles Cellular Types Filaggrin Research Article medicine.medical_specialty Cell Physiology Immunology Materials Science Material Properties Biology Research and Analysis Methods Permeability Dermatitis Atopic Tight Junctions 03 medical and health sciences Signs and Symptoms Diagnostic Medicine medicine Skin equivalent Humans Molecular Biology Techniques Molecular Biology Molecular Biology Assays and Analysis Techniques Tight Junction Proteins Epidermis (botany) Tumor Necrosis Factor-alpha Interleukins lcsh:R Biology and Life Sciences Membrane Proteins Epithelial Cells Cell Biology Molecular Development medicine.disease 030104 developmental biology Biological Tissue Immune System Zonula Occludens-1 Protein Lesions lcsh:Q Interleukin-4 Epidermis Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 9, p e0161759 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Tight junction (TJ) dysfunction in the stratum granulosum leads to aberrant barrier function of the stratum corneum (SC) in the epidermis. However, it is unclear whether TJs are perturbed in atopic dermatitis (AD), a representative aberrant SC-related skin disease, and whether some factors related to AD pathogenesis induce TJ dysfunction. To address these issues, we investigated the alterations of TJs in AD skin and the effects of Th2 and Th17 cytokines on TJs in a skin-equivalent model. The levels of TJ proteins were determined in the epidermis of nonlesional and lesional skin sites of AD. Western blot and immunohistochemical analyses revealed that the levels of zonula occludens 1 were decreased in the nonlesional sites of AD, and the levels of zonula occludens 1 and claudin-1 were decreased in the lesional sites relative to the levels in skin from healthy subjects. Next, we examined the effects of interleukin (IL)-4, tumor necrosis factor-α, IL-17, and IL-22 on the TJ barrier in a skin-equivalent model. Only IL-17 impaired the TJ barrier. Furthermore, we observed a defect in filaggrin monomer degradation in the IL-17-treated skin model. Thus, TJs are dysfunctional in AD, at least partly, due to the effect of IL-17, which may result in an aberrant SC barrier. |
| Databáze: | OpenAIRE |
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