The effects of adenosine and guanosine on the replication of sindbis and vesicular stomatitis viruses in aedes albopictus cells
| Autor: | Victor Stollar, Frank Malinoski |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Adenosine
Ribonucleotide Cytidine Triphosphate viruses Guanosine Uridine Triphosphate Biology Virus Replication Vesicular stomatitis Indiana virus Cell Line chemistry.chemical_compound Vesicular Stomatitis Aedes Virology medicine Animals Nucleotide Uridine chemistry.chemical_classification Molecular biology chemistry Biochemistry Viral replication Sindbis Virus Intracellular medicine.drug |
| Zdroj: | Virology. 115:57-66 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(81)90088-x |
| Popis: | Adenosine and guanosine (100 to 500 μg) inhibited the growth of Aedes albopictus cells and prevented the replication of Sindbis and vesicular stomatitis viruses in these cells. Uridine reversed the effects of adenosine but not of guanosine; actinomycin (0.2 μg/ml) reversed the effects of both adenosine and guanosine on virus replication. Analysis of intracellular nucleotides by thin-layer chromatography and HPLC showed that the levels of CTP and UTP were reduced by both adenosine and guanosine, and that guanosine also markedly reduced the concentration of ATP. The addition of exogenous uridine prevented the reduction in UTP and CTP but had no effect on the guanosine-induced reduction in ATP. The decrease in CTP and UTP seen in adenosine- or guanosine-treated cells and the decrease in ATP seen in the latter were reversed by actinomycin. These results along with earlier work from our laboratory on the antiviral effects of ribavirin suggest that in A. albopictus cells each of the four ribonucleotide triphosphates must be present at a certain minimal concentration in order for virus replication to occur; when the concentration of one or more of these nucleotides falls below a threshold level, viral replication is inhibited. |
| Databáze: | OpenAIRE |
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