Down-regulation of Histone Deacetylases Stimulates Adipocyte Differentiation
| Autor: | Sung Sik Choe, Jun-Jae Chung, Eung Jae Yoo, Jae Bum Kim, Kang Ho Kim |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Down-Regulation
Histone Deacetylase 1 Biochemistry Histone Deacetylases Histones Mice chemistry.chemical_compound Adipocytes Animals Enzyme Inhibitors Promoter Regions Genetic Molecular Biology Histone deacetylase 5 Adipogenesis HDAC11 Histone deacetylase 2 HDAC10 Cell Differentiation Sodium butyrate 3T3 Cells Cell Biology Molecular biology HDAC4 Histone Deacetylase Inhibitors PPAR gamma chemistry Histone deacetylase activity Histone deacetylase |
| Zdroj: | Journal of Biological Chemistry. 281:6608-6615 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m508982200 |
| Popis: | Specific cell type differentiation is driven by programmed regulation of gene expression, which is the result of coordinated modulation of the transcription machinery and chromatin-remodeling factors. We present evidence here that the down-regulation of histone deacetylases is an important process during adipocyte differentiation. In 3T3-L1 cells, histone hyperacetylation was selectively induced at the promoter regions of adipogenic genes during adipocyte differentiation. Interestingly, this was accompanied by a dramatic decrease in the expression level of several histone deacetylases including HDAC1, -2, and -5 and a reduction in overall histone deacetylase enzyme activity. Inhibition of histone deacetylase activity using sodium butyrate resulted in stimulation of adipogenic gene expression and adipocyte differentiation. Consistently, HDAC1 knock-down promoted adipogenesis whereas HDAC1 overexpression attenuated adipocyte differentiation in 3T3-L1 cells. Together, these results suggest that the regulation of not only adipogenic transcription factors, but also chromatin-modifying enzymes is crucial for the execution of bona fide adipogenesis. |
| Databáze: | OpenAIRE |
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