Early changes in synaptic and intrinsic properties of dentate gyrus granule cells in a mouse model of Alzheimer's disease neuropathology and atypical effects of the cholinergic antagonist atropine
Autor: | Elissavet Chartampila, Chiara Criscuolo, Helen E. Scharfman, David Alcantara-Gonzalez |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Atropine
0301 basic medicine Muscarinic receptors Mice Transgenic Hyperexcitability Muscarinic Antagonists Hippocampal formation Inhibitory postsynaptic potential Synaptic Transmission Article lcsh:RC321-571 Mice 03 medical and health sciences 0302 clinical medicine Alzheimer Disease Muscarinic acetylcholine receptor medicine Animals Humans Synaptic potentials lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Intrinsic properties Neurons Chemistry Dentate gyrus Excitatory Postsynaptic Potentials Acetylcholine Disease Models Animal 030104 developmental biology Neurology Dentate Gyrus Excitatory postsynaptic potential NMDA receptor Cholinergic Neuroscience 030217 neurology & neurosurgery Synaptic currents medicine.drug |
Zdroj: | Neurobiology of Disease, Vol 152, Iss, Pp 105274-(2021) Neurobiol Dis |
Popis: | It has been reported that hyperexcitability occurs in a subset of patients with Alzheimer's disease (AD) and hyperexcitability could contribute to the disease. Several studies have suggested that the hippocampal dentate gyrus (DG) may be an important area where hyperexcitability occurs. Therefore, we tested the hypothesis that the principal DG cell type, granule cells (GCs), would exhibit changes at the single-cell level which would be consistent with hyperexcitability and might help explain it. We used the Tg2576 mouse, where it has been shown that hyperexcitability is robust at 2–3 months of age. GCs from 2 to 3-month-old Tg2576 mice were compared to age-matched wild type (WT) mice. Effects of muscarinic cholinergic antagonism were tested because previously we found that Tg2576 mice exhibited hyperexcitability in vivo that was reduced by the muscarinic cholinergic antagonist atropine, counter to the dogma that in AD one needs to boost cholinergic function. The results showed that GCs from Tg2576 mice exhibited increased frequency of spontaneous excitatory postsynaptic potentials/currents (sEPSP/Cs) and reduced frequency of spontaneous inhibitory synaptic events (sIPSCs) relative to WT, increasing the excitation:inhibition (E:I) ratio. There was an inward NMDA receptor-dependent current that we defined here as a novel synaptic current (nsC) in Tg2576 mice because it was very weak in WT mice. Intrinsic properties were distinct in Tg2576 GCs relative to WT. In summary, GCs of the Tg2576 mouse exhibit early electrophysiological alterations that are consistent with increased synaptic excitation, reduced inhibition, and muscarinic cholinergic dysregulation. The data support previous suggestions that the DG contributes to hyperexcitability and there is cholinergic dysfunction early in life in AD mouse models. |
Databáze: | OpenAIRE |
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