Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction
| Autor: | Xinjing Tang, Xinli Fan, Nannan Jing, Lijia Yu, Qian Wang, Han Zhao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Small interfering RNA
RISC complex 010405 organic chemistry Chemistry Ultraviolet Rays Organic Chemistry Gene Expression Aptamers Nucleotide 010402 general chemistry Ligand (biochemistry) Ligands Photochemical Processes 01 natural sciences Biochemistry 0104 chemical sciences Cell biology RNA interference Gene expression Molecular Medicine Gene silencing Gene Silencing RNA Small Interfering Receptor Molecular Biology Gene |
| Zdroj: | Chembiochem : a European journal of chemical biology. 22(11) |
| ISSN: | 1439-7633 |
| Popis: | Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes. |
| Databáze: | OpenAIRE |
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