Candida species biotypes in the oral cavity of infants and children with orofacial clefts under surgical rehabilitation
| Autor: | Jeferson Júnior da Silva, Hudson de Almeida, Thaísla Andrielle da Silva, Cláudio Daniel Cerdeira, José Francisco Höfling, Manoel Francisco Rodrigues Netto, Marcelo Fabiano Gomes Boriollo |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Antifungal Agents Cleft Lip 030106 microbiology Virulence Microbial Sensitivity Tests Oral cavity Microbiology 03 medical and health sciences 0302 clinical medicine Drug Resistance Fungal Amphotericin B Internal medicine medicine Humans Colonization Child Mycological Typing Techniques Candida Mouth business.industry Incidence (epidemiology) Candidiasis Infant 030206 dentistry Corpus albicans Infectious Diseases Nystatin Child Preschool Female business Fluconazole medicine.drug |
| Zdroj: | Microbial Pathogenesis. 124:203-215 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2018.08.042 |
| Popis: | Patients with orofacial clefts present various risk factors for oral infectious diseases, resulting from anatomical and physiological changes and those resulting from rehabilitating therapeutic interventions. The incidence of Candida species in groups of babies and children with orofacial clefts, during pre- and post-operative periods and until return to first consultation, and the profiles for antifungal sensitivity and virulence in vitro were investigated. Oral samples were collected at different times over the surgical procedures and post-surgical clinical consultation and seeded in chromogenic culture media CHROMagar Candida®. Candida biotypes were identified by accessing species-specific genomic DNA sequences by PCR techniques and electrophoretic procedures. Antifungal susceptibility testing was performed by the method of microdilution in broth using the antifungals amphotericin B (AP), nystatin (NYS) and fluconazole (FLC). SAP and PL exoenzyme activities were determined by classical microbiological methods. Some orofacial clefts occurred preferentially in male or female. Low incidence (39.1%) of oral colonization by Candida species (C. albicans, C. krusei, C. tropicalis and Candida spp.) was reported in patient admission to surgical ward, with no correlation to orofacial cleft types or surgical history. Significant reduction in frequencies of Candida and changes of species, over sampling periods, showed dynamic patterns of oral colonization: elimination, maintenance or neocolonization of the biotypes. These biotypes showed sensitivity to AP (100%), partial resistance to FLC ( |
| Databáze: | OpenAIRE |
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