Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery
| Autor: | Bengt Nordén, Kristina Fant, Per Lincoln |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Thermodynamic equilibrium Genetic Vectors 02 engineering and technology Gene delivery 010402 general chemistry Models Biological 01 natural sciences Biochemistry chemistry.chemical_compound Ethidium Dendrimer DNA Packaging Staining and Labeling Oligonucleotide Gene Transfer Techniques Titrimetry DNA Models Theoretical 021001 nanoscience & nanotechnology 0104 chemical sciences Chromatin Folding (chemistry) Kinetics chemistry Biophysics PEGylation Nucleic Acid Conformation 0210 nano-technology |
| Zdroj: | Biochemistry |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi1015887 |
| Popis: | Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic equilibrium. Moreover, dendrimer surface modification through PEGylation appears to make the interaction with DNA more reversible, which is favorable from the perspective of vector unpacking. Probing the nonequilibrium state of DNA during condensation processes is thus important for developing novel vectors, and further, it could also be useful in the study of chromatin folding. |
| Databáze: | OpenAIRE |
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