Chemical Tools for the Study of Intramembrane Proteases

Autor:	Minh T.N. Nguyen, Tim Van Kersavond, Steven H. L. Verhelst
Rok vydání:	2015
Předmět:	Models Molecular Proteases Binding Sites Molecular Structure Cell Membrane General Medicine Biology Biochemistry Transmembrane protein Cell membrane medicine.anatomical_structure Molecular Probes Peptide Hydrolases medicine Humans Molecular Medicine Protease Inhibitors
Zdroj:	ACS Chemical Biology. 10:2423-2434
ISSN:	1554-8937 1554-8929
Popis:	Intramembrane proteases (IMPs) reside inside lipid bilayers and perform peptide hydrolysis in transmembrane or juxtamembrane regions of their substrates. Many IMPs are involved in crucial regulatory pathways and human diseases, including Alzheimer's disease, Parkinson's disease, and diabetes. In the past, chemical tools have been instrumental in the study of soluble proteases, enabling biochemical and biomedical research in complex environments such as tissue lysates or living cells. However, IMPs place special challenges on probe design and applications, and progress has been much slower than for soluble proteases. In this review, we will give an overview of the available chemical tools for IMPs, including activity-based probes, affinity-based probes, and synthetic substrates. We will discuss how these have been used to increase our structural and functional understanding of this fascinating group of enzymes, and how they might be applied to address future questions and challenges.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4f177f6a398c05c19174ee3fe63aeec https://doi.org/10.1021/acschembio.5b00693 Zobrazit plný text záznamu