Removal of damaged proteins during ES cell fate specification requires the proteasome activator PA28
| Autor: | Marija Cvijovic, Åsa Fredriksson, Thomas Nyström, Maria Liljevald, Karin Norrman, Henrik Semb, John Wiseman, Malin Hernebring |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Proteasome Endopeptidase Complex
Protein subunit Cellular differentiation Biology Cell fate determination Article Cell Line Protein Carbonylation 03 medical and health sciences Mice 0302 clinical medicine Gene expression Animals Cell Lineage Embryonic Stem Cells 030304 developmental biology 0303 health sciences Multidisciplinary Activator (genetics) Proteins Cell Differentiation NFKB1 Embryonic stem cell Cell biology Protein Subunits Biochemistry Proteasome 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In embryonic stem cells, removal of oxidatively damaged proteins is triggered upon the first signs of cell fate specification but the underlying mechanism is not known. Here, we report that this phase of differentiation encompasses an unexpected induction of genes encoding the proteasome activator PA28 alpha beta (11S), subunits of the immunoproteasome (20Si), and the 20Si regulator TNF alpha. This induction is accompanied by assembly of mature PA28-20S(i) proteasomes and elevated proteasome activity. Inhibiting accumulation of PA28 alpha using miRNA counteracted the removal of damaged proteins demonstrating that PA28 alpha beta has a hitherto unidentified role required for resetting the levels of protein damage at the transition from self-renewal to cell differentiation. |
| Databáze: | OpenAIRE |
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