Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins
| Autor: | M.M. Elaoufi, W. Benchiha, Zoheir Mehdadi, Ali Latreche, K. Bouterfas |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DPPH Flavonoid Pharmaceutical Science Antioxidants 03 medical and health sciences chemistry.chemical_compound Phenols Picrates Botany Caffeic acid Food science Gallic acid Pharmacology chemistry.chemical_classification Flavonoids 030109 nutrition & dietetics biology Geography Plant Extracts Biphenyl Compounds food and beverages Catechin Marrubium vulgare Free Radical Scavengers Ascorbic acid biology.organism_classification Plant Leaves chemistry Phytochemical Algeria Marrubium |
| Zdroj: | Annales pharmaceutiques francaises. 74(6) |
| ISSN: | 0003-4509 |
| Popis: | To elucidate the effect of the sampling location of Marrubium vulgare L. leaves on phenolic contents and antioxidant proprieties of flavonoids extracts.M. vulgare L. leaves were collected from three different geographical locations belonging to northwest Algeria: Tessala (mountain region), M'sila forest (coastal region), and Ain Skhouna (steppe region). The flavonoid extraction was achieved using organic solvents with different polarities (methanol, chloroform, ethyl acetate, and hexane). Folin-Ciocalteu colorimetric method was used for quantification of total phenolic contents, and aluminum chloride assay for quantification of total flavonoid contents. The antioxidant properties of flavonoids extract were studied by free l,l-diphenyl-2-picrylhydrazyl radical-scavenging technique.Total phenolic and flavonoids concentrations varied respectively between 40.7 and 160mg gallic acid equivalents/g and 27.4 and 66.3mg catechin equivalents/g. The DPPH free radical-scavenging activity shows that the antioxidant activity of the flavonoid extracts varied significantly (P0.001) depending on the type of the organic solvent used, and the sampling location. The methanol, chloroform and ethyl acetate extracts exhibited the highest percentages of inhibition unlike to the aqueous and hexane extracts. These percentages are ranged from 54.8 to 98.8% at 1000μg/mL. In general, M'sila forest flavonoids extracts showed the highest free radical inhibition capacity; followed by those of Ain Skhouna and Tessala Mountain. The inhibitory concentration 50 (ICThe results suggested a potent antioxidant activity of M. vulgare flavonoids extracts, which may find its application in feature research for the food and the pharmacological industries. |
| Databáze: | OpenAIRE |
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