Effects of Croton rhamnifolioides essential oil on Aedes aegypti oviposition, larval toxicity and trypsin activity
| Autor: | Kamilla A. Dutra, Daniela Maria do Amaral Ferraz Navarro, Thiago Henrique Napoleão, Claudia A. Maranhão, Sofia Suely Ferreira Brandão, Geanne K.N. Santos, Bheatriz N. Lima, Camila Soledade de Lira, Patrícia Maria Guedes Paiva |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Aedes aegypti
Croton Oil Oviposition Pharmaceutical Science Gas Chromatography-Mass Spectrometry Article essential oil Analytical Chemistry law.invention lcsh:QD241-441 Toxicology storage lcsh:Organic chemistry law Aedes Drug Discovery parasitic diseases medicine Oils Volatile Animals oviposition deterrent Trypsin Physical and Theoretical Chemistry Trypsin activity Essential oil Larva biology Croton rhamnifolioides Organic Chemistry fungi biology.organism_classification Croton Plant Leaves Mechanism of action Chemistry (miscellaneous) Toxicity Molecular Medicine Composition (visual arts) Female medicine.symptom |
| Zdroj: | Molecules Volume 19 Issue 10 Pages 16573-16587 Molecules, Vol 19, Iss 10, Pp 16573-16587 (2014) |
| ISSN: | 1420-3049 |
| Popis: | Although numerous reports are available concerning the larvicidal potential of essential oils, very few investigations have focused on their mechanisms of action. In the present study, we have investigated the chemical composition of the leaf oil of Croton rhamnifolioides during storage and its effects on oviposition and survival of larvae of the dengue fever mosquito Aedes aegypti. In addition, we have established a possible mechanism of action for the larvicidal activity of the essential oil. GC-MS analyses revealed marked differences in the composition of oil that had been freshly isolated and that of a sample that had been stored in a sealed amber-glass vial under refrigeration for three years. However, both fresh and stored oil exhibited substantial larvicidal activities with LC50 values of 122.35 and 89.03 ppm, respectively, and oviposition deterrent effects against gravid females at concentrations of 50 and 100 µg·mL−1. These results demonstrate that the larvicidal effect of the essential oil was unchanged during three years of storage even though its chemical composition altered. Hence, the essential oil could be used in the preparation of commercial products. In addition, we observed that the trypsin-like activity of mosquito larvae was inhibited in vitro by the essential oil of C. rhamnifolioides, suggesting that the larvicidal effect may be associated with inhibition of this enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|