A novel epitope tag for the detection of rabGTPases
| Autor: | R. Leijendekker, Peter van der Sluijs, Lisya Gerez, Bas Nagelkerken, Marcel van Raak, Karin Mohrmann |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.drug_class
Clinical Biochemistry Guinea Pigs Molecular Sequence Data Hemagglutinin Glycoproteins Influenza Virus GTPase CHO Cells Biology Monoclonal antibody Biochemistry Epitope Analytical Chemistry Mice GTP-Binding Proteins Cricetinae medicine Animals Humans DNA Primers rab5 GTP-Binding Proteins Linear epitope Base Sequence rab4 GTP-Binding Proteins Transfection Molecular biology Guanine Nucleotides Cell biology Vesicular transport protein Cytoplasm Mutation Epitopes B-Lymphocyte Rab Rabbits HeLa Cells |
| Zdroj: | Electrophoresis. 18(14) |
| ISSN: | 0173-0835 |
| Popis: | Rab GTPases are localized on the cytoplasmic surface of most intracellular organelles where they play a role in the regulation of vesicular transport. As it has been difficult to detect endogenous rab proteins by morphological methods, their localizations were often inferred from transfection experiments using epitope-tagged constructs. Because most of the available epitope tags are only recognitzed by mouse monoclonal antibodies they are often not suitable for double or triple label immunocytochemistry. To overcome this problem, we generated antibodies against a novel 10 amino acid X31 influenza hemagglutin epitope (NH). We here characterized these antibodies and document their utility for detecting early endosomal rab proteins N-terminally tagged with the NH decapeptide in morphological and biochemical assays. |
| Databáze: | OpenAIRE |
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