Characterization of FliL Proteins in Bradyrhizobium diazoefficiens : Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System
| Autor: | Michio Homma, Jonathan D. Partridge, Carolina Dardis, Florencia Mengucci, Seiji Kojima, Aníbal R. Lodeiro, Juan Ignacio Quelas, María Julia Althabegoiti, Elías J. Mongiardini |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
FliL
Swarming (honey bee) Motility Flagellum Microbiology Bradyrhizobium 03 medical and health sciences Bacterial Proteins Molecular Biology Gene Bradyrhizobium diazoefficiens Phylogeny Ciencias Exactas Swimming 030304 developmental biology 0303 health sciences biology 030306 microbiology Alphaproteobacteria Membrane Proteins Gene Expression Regulation Bacterial biology.organism_classification Cell biology Regulon Flagella Mutation bacteria Research Article |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP J Bacteriol |
| Popis: | Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each system, more than 30 genes encode the flagellar proteins, most of which are well characterized. Among the exceptions is FliL, which has been scarcely studied in alphaproteobacteria and whose function in other bacterial classes is somewhat controversial. Because each B. diazoefficiens flagellar system contains its own fliL paralog, we obtained the respective deletions ΔfliLS (subpolar) and ΔfliLL (lateral) to study their functions in swimming. We determined that FliLL was essential for lateral flagellum-driven motility. FliLS was dispensable for swimming in either liquid or semisolid medium; however, it was found to play a crucial role in upregulation of the lateral flagellum regulon under conditions of increased viscosity/flagellar load. Therefore, although FliLS seems to be not essential for swimming, it may participate in a mechanosensor complex that controls lateral flagellum induction Instituto de Biotecnologia y Biologia Molecular |
| Databáze: | OpenAIRE |
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