In Vitro Effect of Copper (I) Complex [Cu(NN1)2](ClO4) on Vibrio harveyi BB170 Biofilm Formation
| Autor: | Carla Lozano, Juan Guerrero, Sarita Soto-Aguilera, Brenda Modak, Claudia Lefimil, Mick Parra, Maialen Aldabaldetrecu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
copper (I) complex Minimum bactericidal concentration biology Vibrio harveyi QH301-705.5 Biofilm chemistry.chemical_element biology.organism_classification Antimicrobial Microbiology Copper coumarin biofilm Minimum inhibitory concentration chemistry antibacterial activity Virology antibiofilm activity Biology (General) Antibacterial activity Bacteria Nuclear chemistry |
| Zdroj: | Microorganisms Volume 9 Issue 11 Microorganisms, Vol 9, Iss 2273, p 2273 (2021) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9112273 |
| Popis: | Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN1)2]ClO4, synthesized with Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in Vibrio harveyi BB170 used as model bacteria. Antibacterial activity was measured in vitro by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and half-maximal inhibitory concentration (IC50) determination. Antibiofilm capacity of copper (I) complex was analyzed by different concentrations of IC50 values. The results showed that the sub-IC50 concentration, 12.6 µg/mL of the copper (I) complex, was able to reduce biofilm formation by more than 75%, and bacterial viability was reduced by 50%. Inverted and confocal laser scanning microscopy showed that the [Cu(NN1)2]ClO4 complex affected the biofilm structure. Therefore, the copper (I) complex is effective as an antibiofilm compound in V. harveyi BB170. |
| Databáze: | OpenAIRE |
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