Identification of microRNAs and Their Expression in Leaf Tissues of Guava (Psidium guajava L.) under Salinity Stress
| Autor: | Luis M. Ruiz-Manriquez, Sujay Paul, Ashutosh Sharma, Aashish Srivastava, Francisco I Serrano-Cano, Ana Karen Hernández Aros, Cynthia Karina Tovar Alfaro, Paula Roxana Reyes-Pérez, Yulissa Esmeralda Barrón Andrade |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Regulation of gene expression Genetics 0303 health sciences Psidium biology microRNA computational identification guava Morphogenesis lcsh:S RNA biology.organism_classification 01 natural sciences Stress Response Signaling lcsh:Agriculture 03 medical and health sciences Metabolic pathway Seedling gene regulation Agronomy and Crop Science 030304 developmental biology 010606 plant biology & botany salinity stress |
| Zdroj: | Agronomy, Vol 10, Iss 1920, p 1920 (2020) |
| ISSN: | 2073-4395 |
| Popis: | Superfruit guava (Psidium guajava L.) is one of the healthiest fruits due to its high antioxidant dietary fiber and vitamin content. However, the growth and development of this plant are severely affected by salinity stress, mostly at the seedling stage. MicroRNAs (miRNAs) are small, noncoding, endogenous, highly conserved RNA molecules that play key regulatory roles in plant development, organ morphogenesis, and stress response signaling. In this study, applying computational approaches and following high stringent filtering criteria, a total of 40 potential microRNAs belonging to 19 families were characterized from guava. The identified miRNA precursors formed stable stem-loop structures and exhibited high sequence conservation among diverse and evolutionarily distant plant species. Differential expression pattern of seven selected guava miRNAs (pgu-miR156f-5p, pgu-miR160c-5p, pgu-miR162-3p, pgu-miR164b-5p, pgu-miR166t, pgu-miR167a-5p, and pgu-miR390b-5p) were recorded under salinity stress and pgu-miR162-3p, pgu-miR164b-5p as well as pgu-miR166t were found to be the most affected ones. Using the psRNATarget tool, a total of 49 potential target transcripts of the characterized guava miRNAs were identified in this study which are mostly involved in metabolic pathways, cellular development, and stress response signaling. A biological network has also been constructed to understand the miRNA mediated gene regulation using the minimum free energy (MFE) values of the miRNA-target interaction. To the best of our knowledge, this is the first report of guava miRNAs and their targets. |
| Databáze: | OpenAIRE |
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