| Autor: |
Shufeng Yang, Shaoguang Sui, Yuanhua Qin, Haibo Chen, Shanshan Sha, Xin Liu, Guoying Deng, Yufang Ma |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
APMIS : acta pathologica, microbiologica, et immunologica ScandinavicaREFERENCES. 130(3) |
| ISSN: |
1600-0463 |
| Popis: |
Mycobacterium tuberculosis (M. tuberculosis) Rv1002c encodes the protein O-mannosyltransferase (PMT), which catalyzes the transfer of mannose to serine or threonine residues of proteins. We explored the function of PMT in vitro and in vivo. Rv1002c protein was heterogeneously overexpressed in nonpathogenic Mycobacterium smegmatis (named as MS_Rv1002c). A series of trials including mass spectrometry, transmission electron microscope, biofilm formation and antibiotics susceptibility were performed to explore the function of PMT on bacterial survival in vitro. Mouse experiments were carried out to evaluate the virulence of PMT in vivo. PMT decreased the cell envelope permeability and promoted microbial biofilm formation. PMT enhanced the mycobacterial survival in vivo and inhibited the release of pro-inflammatory cytokines in serum. The function might be associated with an increased abundance of some mannoproteins in culture filtrate (CF). PMT is likely to be involved in mycobacterial survival both in vivo and in vitro due to increasing the mannoproteins abundance in CF. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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