Involvement of IGF-1R-PI3K-AKT-mTOR pathway in increased number of GnRH3 neurons during androgen-induced sex reversal of the brain in female tilapia
| Autor: | Sakura Inoue, Yung Che Tseng, Yasuto Narita, Toyoji Kaneko, Ritsuko Ohtani-Kaneko, Suzuka Shiono, Akari Oda, Ryo Kaneko |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty food.ingredient medicine.drug_class Biology Receptor IGF Type 1 Gonadotropin-Releasing Hormone Phosphatidylinositol 3-Kinases Text mining food Internal medicine Methyltestosterone medicine Animals Testosterone Insulin-Like Growth Factor I Protein kinase B PI3K/AKT/mTOR pathway Neurons Multidisciplinary business.industry Reproduction TOR Serine-Threonine Kinases Brain Tilapia Sex reversal Androgen Pyrrolidonecarboxylic Acid Endocrinology Female business Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Scientific reports. 12(1) |
| ISSN: | 2045-2322 |
| Popis: | The neuroplastic mechanism of sex reversal in the fish brain remains unclear due to the difficulty in identifying the key neurons involved. Mozambique tilapia show different reproductive behaviours between sexes; males build circular breeding nests while females hold and brood fertilized eggs in their mouth. In tilapia, gonadotropin-releasing hormone 3 (GnRH3) neurons, located in the terminal nerve, regulate male reproductive behaviour. Mature males have more GnRH3 neurons than mature females, and these neurons have been indicated to play a key role in the androgen-induced female-to-male sex reversal of the brain. We aimed to elucidate the signalling pathway involved in the androgen-induced increase in GnRH3 neurons in mature female tilapia. Applying inhibitors to organotypic cultures of brain slices, we showed that the insulin-like growth factor (IGF)-1 receptor (IGF-1R)/PI3K/AKT/mTOR pathway contributed to the androgen-induced increase in GnRH3 neurons. The involvement of IGF-1 and IGF-1R in 11-ketotestosterone (11-KT)-induced development of GnRH3 neurons was supported by an increase in Igf-1 mRNA shortly after 11-KT treatment, the increase of GnRH3 neurons after IGF-1 treatment and the expression of IGF-1 in GnRH3 neurons. Our findings highlight the involvement of IGF-1 and its downstream signalling pathway in the sex reversal of the tilapia brain. |
| Databáze: | OpenAIRE |
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