Emergent Properties of Reduced-Genome Escherichia coli
| Autor: | David Frisch, Guy Plunkett, Shamik S. Sharma, Buffy Stahl, György Pósfai, Vitaliy Kolisnychenko, Kinga Umenhoffer, Günther M. Keil, Monika de Arruda, Valerie Burland, Tamás Fehér, Frederick R. Blattner, Sarah W. Harcum |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Genetics Mutation Multidisciplinary Escherichia coli K12 Mutagenesis (molecular biology technique) Virulence Biology medicine.disease_cause Genome Synthetic biology Plasmid Species Specificity Mutagenesis DNA Transposable Elements medicine Genetic Engineering Escherichia coli Gene Gene Deletion Genome Bacterial Plasmids |
| Zdroj: | Science. 312:1044-1046 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.1126439 |
| Popis: | With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving good growth profiles and protein production. Genome reduction also led to unanticipated beneficial properties: high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains. Eradication of stress-induced transposition evidently stabilized the MDS genomes and provided some of the new properties. |
| Databáze: | OpenAIRE |
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