Investigation of the reliability of 24 h urine excretion as a biomarker of isoflavone exposure over time and over a wide range of isoflavone intakes
| Autor: | John H. Cummings, A. Blake, Nigel Deighton, C. M. Steel, A. M. Thompson, Michael S. Morton, Margaret R. Ritchie |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
medicine.medical_specialty Time Factors Urinary system Medicine (miscellaneous) Genistein Urine Sensitivity and Specificity Excretion chemistry.chemical_compound Internal medicine medicine Humans 24 h urine Cross-Over Studies Nutrition and Dietetics business.industry Daidzein Reproducibility of Results food and beverages Middle Aged Isoflavones Endocrinology chemistry Dietary Supplements Biomarker (medicine) Female business Biomarkers |
| Zdroj: | European Journal of Clinical Nutrition. 58:1286-1289 |
| ISSN: | 1476-5640 0954-3007 |
| DOI: | 10.1038/sj.ejcn.1601963 |
| Popis: | Objective: To study the variation in genistein+daidzein intake over a 6-month period and test the reliability of 24 h urinary isoflavones as a biomarker of exposure over time. Design: Dietary genistein+daidzein intake was assessed at various time points throughout six months in 15 healthy subjects. Group 1 (n=8) followed nonsupplemented diets and Group 2 (n=7) took a 35 mg/d isoflavone supplement for 3 months and each subject provided a 24 h urine collection, validated with para-aminobenzoic acid, during weeks 7, 15 and 19. Urine was analysed for genistein and daidzein using LC-MS. Results: Isoflavone intake in Groups 1 and 2 ranged from 0.00 to 1.1 mg/d and 0.1 to 53.1 mg/d, respectively. Urine excretion for both groups ranged from 0.20 to 9.56 mg/d. The relationship between 24 h excretion and isoflavone intake is y=0.44 × ±0.03(standard deviation) + 1.57; r=0.89, P |
| Databáze: | OpenAIRE |
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