| Autor: |
David M. Arana, Yolanda Hernández-Hermida, Juana Cacho-Calvo |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Enfermedades infecciosas y microbiologia clinica (English ed.). |
| ISSN: |
2529-993X |
| Popis: |
This study proposes a simple and rapid method for both bacterial identification and direct antimicrobial susceptibility testing (AST) by using MALDI-TOF and a double differential centrifugation-wash procedure from positive blood cultures.Fifty-two positive blood cultures (37 gramnegative bacilli and 15 grampositive cocci) were studied by two methods for identification and AST: a reference method, and the rapid MALDI-TOF method obtaining a purified pellet by using a double differential centrifugation procedure.A total of 1101 MIC values (mg/l) were interpreted according to EUCAST clinical breakpoints and compared using the two methods simultaneously. Discrepancies in 81 MIC values (7.35%) were detected. By analyzing standard parameters, we obtained 98.28% essential agreement and 92.65% categorical agreement considering all isolates tested.This method provides rapid bacterial identification and AST, offering definitive results 24-48h earlier than the conventional method (p0.001) and improving the turnaround time in blood culture diagnostics, especially in laboratories without 24-h on-call. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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