Basic Techniques in Mammalian Cell Tissue Culture
| Autor: | Kristin M. May, Katy Phelan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Antifungal Agents Cell Survival Primary Cell Culture Cell Culture Techniques Cell Count Transportation Biology Toxicology Tissue Culture Techniques 03 medical and health sciences Tissue culture 0302 clinical medicine Mammalian cell Freezing Animals Humans Trypsin Cells Cultured Cryopreservation Mammals Staining and Labeling Cell Biology Anti-Bacterial Agents Culture Media Cell biology 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Tissue Preservation |
| Zdroj: | Current Protocols in Cell Biology |
| ISSN: | 1934-2616 1934-2500 |
| DOI: | 10.1002/0471143030.cb0101s66 |
| Popis: | Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2016 by John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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