Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor
| Autor: | Zhu Zeng, Huifang Zhang, Yudan Zhang, Jing Chen, Nina Huang, Haixin Ma, Yufei Su |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Human Embryonic Stem Cells Apoptosis Myocardial Reperfusion Injury Protective Agents Cell Line 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Lactate dehydrogenase Calcium flux medicine Animals Humans Myocytes Cardiac Calcium Signaling Receptor Molecular Biology 3' Untranslated Regions biology Antagomirs Feeder Cells Cell Biology Fibroblasts medicine.disease Molecular biology Cell Hypoxia Rats Receptors Neurotransmitter Up-Regulation Oxygen MicroRNAs Oxidative Stress 030104 developmental biology Glucose chemistry 030220 oncology & carcinogenesis Gene Knockdown Techniques biology.protein Creatine kinase Calcium Reactive Oxygen Species Reperfusion injury Neuromedin U Intracellular Developmental Biology Research Paper |
| Zdroj: | Cell Cycle |
| ISSN: | 1551-4005 |
| Popis: | The present study aimed to assess the role of miR-1275 in cardiac ischemia reperfusion injury. H9 human embryonic stem cell (hESC)-derived cardiomyocytes stimulated by oxygen-glucose deprivation/reoxygenation (OGD/R) were used to simulate myocardial injury in vitro. miR-1275 expression levels in cells were measured by RT-qPCR. The release of lactate dehydrogenase (LDH) and creatine kinase (CK) was examined through LDH and CK ELISA kits. Cell apoptosis was detected through flow cytometry. A Fura-2 Calcium Flux Assay Kit and a Fluo-4 assay kit were used to determine the Ca2+ concentration. Expression levels of proteins were tested by Western blotting. The binding effect of miR-1275 and neuromedin U type 1 receptor (NMUR1) was detected by dual-luciferase activity assay. The results showed that miR-1275 was upregulated in OGD/R-stimulated cardiomyocytes. Inhibition of miR-1275 suppressed the increased activity of LDH and CK, cell apoptosis, reactive oxygen species (ROS) production, intracellular Ca2+ concentration and sarcoplasmic reticulum (SR) Ca2+ leak induced by OGD/R treatment in cardiomyocytes. miR-1275 directly targets 3ʹUTR of NMUR1 and negatively regulates NMUR1 expression. Silence of NMUR1 abolished the protecting effect of the miR-1275 antagomir on myocardial OGD/R injury. Our study indicated that the miR-1275 antagomir protects cardiomyocytes from OGD/R injury through the promotion of NMUR1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|