Improved viability and reduced apoptosis in sub-zero 21-hour preservation of transplanted rat hearts using anti-freeze proteins
| Autor: | Aram Smolinsky, Sheick Yousif Basheer, Gabriel Amir, Micha S. Feinberg, Liana Horowitz, Boris Rubinsky, Jacob Lavee, Leor Jonathan |
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| Rok vydání: | 2004 |
| Předmět: |
Pulmonary and Respiratory Medicine
Sarcomeres Pathology medicine.medical_specialty Adenosine Transplantation Heterotopic Cell Survival Allopurinol Organ Preservation Solutions Diastole Hemodynamics Apoptosis Cryopreservation Mitochondria Heart Andrology Rats Sprague-Dawley Raffinose Posterior wall Antifreeze Proteins medicine Animals Insulin Viaspan Transplantation business.industry Heart Rat heart Organ Preservation Glutathione Rats Cold Temperature Echocardiography Heart Transplantation Surgery Cardiology and Cardiovascular Medicine business |
| Zdroj: | The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 24(11) |
| ISSN: | 1557-3117 |
| Popis: | Freeze-tolerant fish survive sub-zero temperatures by non-colligatively lowering the freezing temperature of their body fluids using anti-freeze proteins (AFPs). We sought to evaluate and compare the effects of prolonged sub-zero cryopreservation of transplanted rat hearts using AFP I or AFP III.Two heterotopic rat heart transplantation protocols were used. In Protocol 1 (n = 104), hearts (n = 8/group) were preserved for 12, 18 and 24 hours in University of Wisconsin solution (UW) at 4 degrees C, UW at -1.3 degrees C, UW/AFP I at -1.3 degrees C and UW/AFP III at -1.3 degrees C, with and without nucleation. Post-operative evaluation consisted of visual viability scoring of the hearts after 60 minutes. Protocol 2 (n = 58) involved evaluation of 24-hour post-transplant viability, echocardiography (fractional shortening [FS], left ventricular end-systolic and -diastolic diameter [ESD, EDD] and anterior and posterior wall systolic and diastolic thickness [AWT-S, AWT-D, PWT-S, PWT-D]), TUNEL staining and electron microscopy (EM) findings for hearts preserved for 18, 21 and 24 hours in UW at 4 degrees C or UW/AFP III at -1.3 degrees C.Hearts preserved in UW at -1.3 degrees C with nucleation froze and died. Three of 8 hearts preserved in UW at 4 degrees C for 24 hours died, whereas all hearts preserved at -1.3 degrees C survived. Hearts preserved in UW/AFP for 18 and 24 hours at -1.3 degrees C had superior viability scores compared with those in UW at 4 degrees C. Hearts in AFP III at -1.3 degrees C displayed greater AWT-S and AWT-D (3.5 +/- 0.2 vs 2.4 +/- 0.2, p0.05, and 3.5 +/- 0.2 vs 2.2 +/- 0.2, p0.05, respectively) after 18-hour preservation. In the 21-hour preservation group, AFP-treated hearts displayed improved echocardiographic systolic contraction indices, including: improved FS (27 +/- 3.7 vs 15 +/- 4, p = 0.04); diminished ESD (0.28 +/- 0.57 vs 0.47 +/- 0.6, p0.05); greater AWT-S (3.4 +/- 0.18 vs 2.8 +/- 0.2, p0.05); and fewer positively TUNEL-stained nuclei per specimen (35 +/- 14 vs 5.3 +/- 2.7, p = 0.04). Also, improved EM scores were noted compared with UW at 4 degrees C.In prolonged sub-zero cryopreservation, AFPs protect the heart from freezing, improve survival and hemodynamics, and reduce apoptotic cell death. |
| Databáze: | OpenAIRE |
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