Changes in optical properties of electroporated cells as revealed by digital holographic microscopy
| Autor: | Nicolae Mihale, Eugenia Kovacs, Mihaela G. Moisescu, Tudor Savopol, Violeta L. Calin, Mona Mihailescu, Alexandra V. Baluta |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Electrochemotherapy Materials science business.industry Atomic force microscopy Electroporation 01 natural sciences Atomic and Molecular Physics and Optics Article 010309 optics Cell membrane 03 medical and health sciences 030104 developmental biology Optics medicine.anatomical_structure 0103 physical sciences medicine Projected area Pulse wave Digital holographic microscopy sense organs business Refractive index Biotechnology Biomedical engineering |
| Popis: | Changes in optical and shape-related characteristics of B16F10 cells after electroporation were investigated using digital holographic microscopy (DHM). Bipolar rectangular pulses specific for electrochemotherapy were used. Electroporation was performed in an "off-axis" DHM set-up without using exogenous markers. Two types of cell parameters were monitored seconds and minutes after pulse train application: parameters addressing a specifically defined area of the cell (refractive index and cell height) and global cell parameters (projected area, optical phase shift profile and dry mass). The biphasic behavior of cellular parameters was explained by water and mannitol dynamics through the electropermeabilized cell membrane. |
| Databáze: | OpenAIRE |
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