Transposon-tagging identifies novel pathogenicity genes in Fusarium graminearum

Autor: Taiguo Liu, Gert H. J. Kema, Cees Waalwijk, Sarrah Ben M’Barek, Xu Zhang, Marie-Josée Daboussi, Theo van der Lee, Marie Dufresne, Xiude Xu, Wenwei Zhang
Přispěvatelé: Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2008
Předmět:
0106 biological sciences
Transposable element
family
oxysporum
Transposon tagging
MESH: Triticum
arabidopsis-thaliana
MESH: Virulence
Biology
01 natural sciences
Microbiology
Genome
Insertional mutagenesis
03 medical and health sciences
MESH: Plant Diseases
Fusarium
mediated transformation
Genetics
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Gene
Triticum
Transposase
Plant Diseases
030304 developmental biology
MESH: Fusarium
MESH: Genetic Complementation Test
0303 health sciences
Biointeracties and Plant Health
Virulence
magnaporthe-grisea
Genetic Complementation Test
filamentous fungi
food and beverages
Sleeping Beauty transposon system
inverted-repeat elements
Laboratorium voor Phytopathologie
Mutagenesis
Insertional
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: DNA Transposable Elements
MESH: Mutagenesis
Insertional
Laboratory of Phytopathology
DNA Transposable Elements
insertional mutagenesis
rice blast fungus
PRI Biointeractions en Plantgezondheid
Transposon mutagenesis
genome-wide analysis
010606 plant biology & botany
Zdroj: Fungal Genetics and Biology
Fungal Genetics and Biology, Elsevier, 2008, 45 (12), pp.1552-61. ⟨10.1016/j.fgb.2008.09.004⟩
Fungal Genetics and Biology 45 (2008) 12
Fungal Genetics and Biology, 45(12), 1552-1561
ISSN: 1087-1845
1096-0937
DOI: 10.1016/j.fgb.2008.09.004
Popis: International audience; With the increase of sequenced fungal genomes, high-throughput methods for functional analyses of genes are needed. We assessed the potential of a new transposon mutagenesis tool deploying a Fusarium oxysporum miniature inverted-repeat transposable element mimp1, mobilized by the transposase of impala, a Tc1-like transposon, to obtain knock-out mutants in Fusarium graminearum. We localized 91 mimp1 insertions which showed good distribution over the entire genome. The main exception was a major hotspot on chromosome 2 where independent insertions occurred at exactly the same nucleotide position. Furthermore insertions in promoter regions were over-represented. Screening 331 mutants for sexual development, radial growth and pathogenicity on wheat resulted in 19 mutants (5.7%) with altered phenotypes. Complementation with the original gene restored the wild-type phenotype in two selected mutants demonstrating the high tagging efficiency. This is the first report of a MITE transposon tagging system as an efficient mutagenesis tool in F. graminearum.
Databáze: OpenAIRE
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