Cancer Stem Cell and Embryonic Development-Associated Molecules Contribute to Prognostic Significance in Ovarian Cancer
| Autor: | Muzaffer Sanci, Gulperi Oktem, Sibel Demir Keçeci, Şule Ayla, Yusuf Yildirim, Sevinc Inan, Ayhan Bilir |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Oncology medicine.medical_specialty Pathology Serous carcinoma Carcinoma Ovarian Epithelial Metastasis Cell Line Tumor Spheroids Cellular Internal medicine Biomarkers Tumor medicine Humans Mucinous carcinoma Serrate-Jagged Proteins Neoplasms Glandular and Epithelial Receptor Notch1 Aged Neoplasm Staging Ovarian Neoplasms business.industry Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins Membrane Proteins Obstetrics and Gynecology Cancer Middle Aged Prognosis medicine.disease Adenocarcinoma Mucinous Immunohistochemistry Proto-Oncogene Proteins c-kit Serous fluid embryonic structures Intercellular Signaling Peptides and Proteins Adenocarcinoma Female Mucinous Tumor Neoplasm Grading business Ovarian cancer Carcinoma Endometrioid Jagged-1 Protein |
| Zdroj: | International Journal of Gynecologic Cancer. 22:23-29 |
| ISSN: | 1525-1438 1048-891X |
| Popis: | ObjectivesEmbryonic molecules and cancer stem cell signaling resemble each other, and they organize cancer modality. We hypothesized that similar immunohistochemical expressions between tumor spheroids and patients’ samples compared with clinical relevance would give an important clue in patients’ prognosis.MethodsImmunohistochemical expression of c-kit, Notch1, Jagged1, and Delta1 in 50 cases of primary ovarian tumors (10 endometrioid, 10 serous, 10 mucinous adenocarcinoma, 10 borderline serous, and 10 borderline mucinous tumors) and MDAH-2774 spheroids were investigated. Results were compared in both spheroids and tumor samples with morphologic parameters (histological grade) and clinical data (age, stage, tumor size, and metastasis).ResultsHigh c-kit and Notch1 immunoreactivity was shown in spheroids, but interestingly immunoreactivity of these molecules in tumor samples was different from patients’ clinicopathological characteristics. In serous carcinoma, metastasis correlated with Notch1 immunoexpression; in mucinous carcinoma, Jagged1 immunohistochemistry correlated with grade, stage, and metastasis of tumor; in borderline serous and mucinous tumors, Jagged1 correlated with high grade. Moreover, Jagged1 correlated with stage and Notch1 with size in borderline mucinous tumor. Endometrioid carcinoma statistics showed that there was a correlation between age and Notch1 expression.ConclusionNotch1, Jagged1, and Delta1 expressions might be useful markers for clinical prognosis of ovarian carcinomas; and Notch pathway, one of the most intensively studied putative therapeutic targets, may be a useful marker for cancer. Consequently, Jagged1 could be a marker for tumor grades and Notch1 as a marker for metastases. |
| Databáze: | OpenAIRE |
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