Cloning, sequencing, and promoter identification of Blueberry red ringspot virus, a member of the family Caulimoviridae with similarities to the 'Soybean chlorotic mottle-like' genus
| Autor: | D. C. Ramsdell, James J. Polashock, Bernadette M. Glasheen, J. M. Gillett, Bradley I. Hillman, Diane M. Lawrence, N. Vorsa |
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| Rok vydání: | 2002 |
| Předmět: |
Transcription
Genetic Blueberry Plants Molecular Sequence Data Polymerase Chain Reaction Genome Open Reading Frames Soybean chlorotic mottle virus Caulimovirus Virology Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Gene Genomic organization Cloning Genetics Base Sequence biology Nucleic acid sequence General Medicine biology.organism_classification DNA Viral Capsid Proteins Soybeans Caulimoviridae |
| Zdroj: | Archives of Virology. 147:2169-2186 |
| ISSN: | 0304-8608 |
| DOI: | 10.1007/s00705-002-0866-7 |
| Popis: | The double-stranded DNA genome of Blueberry red ringspot virus (BRRV), a member of the family Caulimoviridae, was cloned and sequenced. The genome organization and relationships of the 8303 nt sequence revealed BRRV to be a tentative member of the genus that has been provisionally named "Soybean chlorotic mottle-like viruses", rather than a member of the genus Caulimovirus, in which it had been placed previously. Insertion of the putative 35S promoter homolog of BRRV into promoterless constructs carrying the UidA (beta-glucuronidase) gene resulted in high-level transient expression from cranberry and stable expression from transgenic tobacco. Sequences of 5'-RACE clones derived from transcripts from transgenic tobacco were consistent with the map position of the promoter. |
| Databáze: | OpenAIRE |
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