Creatine supplementation does not promote tumor growth or enhance tumor aggressiveness in Walker-256 tumor-bearing rats
| Autor: | Philippe B. Guirro, Flávia Alessandra Guarnier, Rubens Cecchini, Paola Sanches Cella, Camila S. Padilha, Mayra T. J. Testa, Rafael Deminice, Poliana Camila Marinello, José Alberto Duarte |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Cellular differentiation 030209 endocrinology & metabolism Apoptosis Creatine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Neoplasms medicine Carcinoma Tumor Microenvironment Animals Tumor growth Carcinoma 256 Walker Rats Wistar Tumor microenvironment 030109 nutrition & dietetics Nutrition and Dietetics Cell growth business.industry T-cell receptor medicine.disease Rats Endocrinology chemistry Dietary Supplements business |
| Zdroj: | Nutrition (Burbank, Los Angeles County, Calif.). |
| ISSN: | 1873-1244 |
| Popis: | Objectives This study aimed to analyze the effect of creatine (Cr) supplementation on tumor microenvironment, evaluating the parameters of tumor aggressiveness. Methods Sixteen male Wistar rats were randomly assigned to 2 groups (n = 8/group): Tumor-bearing (T) and tumor-bearing supplemented with Cr (TCr). Cr supplementation was provided in drinking water for a total of 21 d. After 11 d of Cr supplementation (TCr group) or water (T group), Walker-256 tumor cells were inoculated subcutaneously in the right flank of all rats, which kept receiving Cr supplementation (TCr group) or water (T group) for 10 more days. The total period of the experiment was 21 d. Results Tumor weight corresponded with approximately 3.5% ± 0.9% of animal body weight in the T group. Cr supplementation did not accelerate tumor growth or increase tumor size. The histopathological analysis demonstrated the presence of nuclear pleomorphisms and atypical nuclei, with the presence of low-differentiated tumor cells, in both groups. Cr supplementation did not alter apoptosis and cell proliferation markers, nor tumor capsule thickness and viable tumor area. Conclusions Cr supplementation in Walker-256 tumor-bearing rats did not induce significant changes in tumor development, and did not interfere with the parameters of tumor aggressiveness, such as the level of cell differentiation and proliferation. |
| Databáze: | OpenAIRE |
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