Comparison between the characteristics of follicular fluid and the developmental competence of bovine oocytes
| Autor: | Hisataka Iwata, J. Inoue, Takehito Kuwayama, Takeshi Kuge, Koji Kimura, Yasunori Monji |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Embryonic Development Fertilization in Vitro Biology Fatty Acids Nonesterified Blood Urea Nitrogen Follicle chemistry.chemical_compound Endocrinology NEFA Human fertilization Food Animals Internal medicine Lactate dehydrogenase medicine Animals Magnesium Aspartate Aminotransferases Blood urea nitrogen L-Lactate Dehydrogenase Embryogenesis Embryo Bilirubin General Medicine Blastula Follicular fluid Follicular Fluid chemistry Oocytes Animal Science and Zoology Cattle Female |
| Zdroj: | Animal reproduction science. 91(3-4) |
| ISSN: | 0378-4320 |
| Popis: | There are great differences in the developmental competence of oocytes collected from individual cows. Oocytes grow and mature in the follicular fluid (FF). In the present study, characteristics of the FF of each ovary and the developmental competence of enclosed oocytes were investigated, and these data were then compared. A total of 37 pairs of ovaries were collected from beef heifers. The concentration of magnesium (Mg), aspirate aminotransferase (AST), nonesterified fatty acids (NEFA), and lactate dehydrogenase (LDH) in the FF were great compared with serum standard. Several significant correlations among these characteristics were detected. Forty-eight hours after fertilization, the stage of embryo development at an advanced developmental stage (>6 cell stage) is related to the rate of blastulation 8 days after fertilization. In addition, a significantly positive or negative correlation was observed between the developmental competence (the rate of cleavage in the embryo and blastulation) and the concentration of the icterus index (ICT) or blood urea nitrogen (BUN) in the FF. In conclusion, the quality of oocytes is affected by the environment in the follicle, and BUN or ICT is a predictable index of the developmental competence of oocytes. |
| Databáze: | OpenAIRE |
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