The Streptomycin-Treated Mouse Intestine Selects Escherichia coli envZ Missense Mutants That Interact with Dense and Diverse Intestinal Microbiota
| Autor: | Megan E. Banner, Tyrrell Conway, Jimmy Adediran, Matthew E. Mokszycki, Mary P. Leatham-Jensen, Joyce E. Caughron, Karen A. Krogfelt, Jakob Frimodt-Møller, Paul S. Cohen |
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| Rok vydání: | 2012 |
| Předmět: |
Immunology
Mutant Mutation Missense Biology medicine.disease_cause Microbiology Mice chemistry.chemical_compound Multienzyme Complexes Escherichia coli medicine Animals Selection Genetic Escherichia coli Proteins Biofilm Gene Expression Regulation Bacterial Bacterial Infections Adaptation Physiological Mucus In vitro Intestines Infectious Diseases chemistry Streptomycin Biofilms Galactose Colicin bacteria Parasitology Bacterial Outer Membrane Proteins medicine.drug |
| Zdroj: | Infection and Immunity. 80:1716-1727 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.06193-11 |
| Popis: | Previously, we reported that the streptomycin-treated mouse intestine selected nonmotile Escherichia coli MG1655 flhDC deletion mutants of E. coli MG1655 with improved colonizing ability that grow 15% faster in vitro in mouse cecal mucus and 15 to 30% faster on sugars present in mucus (M. P. Leatham et al., Infect. Immun. 73:8039–8049, 2005). Here, we report that the 10 to 20% remaining motile E. coli MG1655 are envZ missense mutants that are also better colonizers of the mouse intestine than E. coli MG1655. One of the flhDC mutants, E. coli MG1655 Δ flhD , and one of the envZ missense mutants, E. coli MG1655 mot-1, were studied further. E. coli MG1655 mot-1 is more resistant to bile salts and colicin V than E. coli MG1655 Δ flhD and grows ca. 15% slower in vitro in mouse cecal mucus and on several sugars present in mucus compared to E. coli MG1655 Δ flhD but grows 30% faster on galactose. Moreover, E. coli MG1655 mot-1 and E. coli MG1655 Δ flhD appear to colonize equally well in one intestinal niche, but E. coli MG1655 mot-1 appears to use galactose to colonize a second, smaller intestinal niche either not colonized or colonized poorly by E. coli MG1655 Δ flhD . Evidence is also presented that E. coli MG1655 is a minority member of mixed bacterial biofilms in the mucus layer of the streptomycin-treated mouse intestine. We offer a hypothesis, which we call the “Restaurant” hypothesis, that explains how nutrient acquisition in different biofilms comprised of different anaerobes can account for our results. |
| Databáze: | OpenAIRE |
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