Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori
| Autor: | Xiaoguang Liu, Qisheng Song, Mengfang Du, Shiheng An, Shuangyin Han, Xinming Yin, Xiaoming Liu |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Proteomics Time Factors viruses Molecular Sequence Data Real-Time Polymerase Chain Reaction Article chemistry.chemical_compound Biosynthesis Tandem Mass Spectrometry Bombyx mori Animals Amino Acid Sequence Sex Attractants Multidisciplinary biology Neuropeptides fungi Animal Structures Lipid Droplets Bombyx biology.organism_classification Gene Ontology Gene Expression Regulation chemistry Biochemistry Gene Knockdown Techniques Sex pheromone Glycerol-3-Phosphate O-Acyltransferase Insect Proteins Female RNA Interference Glycerophosphate Acyltransferase Fatty Alcohols |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep08110 |
| Popis: | Female moths employ their own pheromone blends as a communicational medium in mating behavior. The biosynthesis and release of sex pheromone in female moths are regulated by pheromone biosynthesis activating neuropeptide (PBAN) and the corresponding action of PBAN has been well elucidated in Bombyx mori. However, very little is known about the molecular mechanism regarding the biosynthesis of sex pheromone precursor. In this study, quantitative proteomics was utilized to comprehensively elucidate the expression dynamics of pheromone glands (PGs) during development. Proteomic analysis revealed a serial of differentially expressed sex pheromone biosynthesis-associated proteins at the different time points of B. mori development. Most interestingly B. mori glycerol-3-phosphate O-acyltransferase (BmGPAT) was found to be expressed during the key periods of sex pheromone biosynthesis. RNAi knockdown of BmGPAT confirmed the important function of this protein in the biosynthesis of sex pheromone precursor, triacylglcerol (TAG) and subsequently PBAN-induced production of sex pheromone, bombykol. Behavioral analysis showed that RNAi knockdown of GPAT significantly impaired the ability of females to attract males. Our findings indicate that GPAT acts to regulate the biosynthesis of sex pheromone precursor, TAG, thus influencing PBAN-induced sex pheromone production and subsequent mating behavior. |
| Databáze: | OpenAIRE |
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