Radachlorin-Containing Microparticles for Photodynamic Therapy

Autor: Anastasia Maksimovna Miroshkina, Maria N. Yakovtseva, E.N. Mochalova, Sergey Petrovich Krechetov, A. V. Babenyshev, Ivan Maslov, Ivan I. Krasnyuk, Alexander A. Loshkarev
Jazyk: angličtina
Rok vydání: 2021
Předmět:
NANOCARRIERS
Scanning electron microscope
medicine.medical_treatment
Pharmaceutical Science
Photodynamic therapy
02 engineering and technology
030226 pharmacology & pharmacy
chemistry.chemical_compound
drug delivery systems
MICROSPHERES
0302 clinical medicine
NANOPARTICLES
Copolymer
Photosensitizer
Pharmacology & Pharmacy
DRUG-DELIVERY
General Pharmacology
Toxicology and Pharmaceutics
skin and connective tissue diseases
microparticles
CHLORIN E6
DERIVATIVES
Singlet oxygen
021001 nanoscience & nanotechnology
Photosensitizing agents
Drug delivery systems
PLGA
photodynamic therapy
0210 nano-technology
Life Sciences & Biomedicine
Research Article
congenital
hereditary
and neonatal diseases and abnormalities
Radachlorin
RM1-950
Microparticles
NANOMEDICINES
radachlorin
03 medical and health sciences
Dynamic light scattering
medicine
TOOLS
Science & Technology
STABILITY
tumor cell line
nutritional and metabolic diseases
Tumor cell line
IN-VITRO
photosensitizing agents
chemistry
Therapeutics. Pharmacology
Nuclear chemistry
Zdroj: Advanced Pharmaceutical Bulletin, Vol 11, Iss 3, Pp 458-468 (2021)
Advanced Pharmaceutical Bulletin
ISSN: 2251-7308
2228-5881
Popis: Purpose: Reducing the undesirable systemic effect of photodynamic therapy (PDT) can be achieved by incorporating a photosensitizer in microparticles (MPs). This study is devoted to the preparation of biocompatible biodegradable MPs with the inclusion of the natural photosensitizer Radachlorin (RС) and an assessment of the possibility of their use for PDT. Methods: RC-containing MPs (RС MPs) with poly(lactic-co-glycolic acid) copolymer (PLGA) matrix were prepared by a double emulsion solvent evaporation methods. The size and morphology of RC MPs were surveyed using scanning electron microscopy, confocal laser scanning microscopy, and dynamic light scattering. The content of RC, its release from RC MPs, and singlet oxygen generation were evaluated by the optical spectroscopy. Cellular uptake and cytotoxic photodynamic effect of RC MPs were investigated with in vitro assays. Results: The average diameter of the prepared RC MPs was about 2-3 μm. The RC MPs prepared by the water/oil/oil method had a significantly higher inclusion of RC (1.74 μg/mg) then RC MPs prepared by the water/oil/water method (0.089 μg/mg). Exposure of the prepared RC MPs to PDT light radiation was accompanied by the singlet oxygen generation and a cytotoxic effect for tumor cells. The release of the RC from the RC MPs was prolonged and lasted at least two weeks. Conclusion: PLGA RC MPs were found to cause a photoactivated cytotoxic effect for tumor cells and can be used for local application in PDT of tumors. ispartof: ADVANCED PHARMACEUTICAL BULLETIN vol:11 issue:3 pages:458-468 ispartof: location:Iran status: published
Databáze: OpenAIRE
Externí odkaz: