Natural infection by Trypanosoma cruzi in triatomines and seropositivity for Chagas disease of dogs in rural areas of Rio Grande do Norte, Brazil
Autor: | Jamille Maia e Magalhães, Yannara Barbosa Nogueira Freitas, Hélcio R. Gil-Santana, Tânia Zaverucha do Valle, Luiz Ney d’Escoffier, Maressa Laíse Reginaldo de Sousa, Celeste da Silva Freitas de Souza, Thais Aaparecida Kazimoto, Sthenia Santos Albano Amora, Teresa Cristina Monte Gonçalves |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Microbiology (medical) Chagas disease Rural Population Veterinary medicine lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Trypanosoma cruzi 030231 tropical medicine Antibodies Protozoan Domestic reservoir Polymerase Chain Reaction Serology law.invention Zoonosis 03 medical and health sciences 0302 clinical medicine Dogs law Diagnosis parasitic diseases medicine Prevalence Animals Chagas Disease Dog Diseases Fluorescent Antibody Technique Indirect Polymerase chain reaction biology 030108 mycology & parasitology biology.organism_classification medicine.disease Triatoma brasiliensis Insect Vectors Infectious Diseases Vector (epidemiology) Protozoa Parasitology Vector Triatominae Brazil |
Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Volume: 51, Issue: 2, Pages: 190-197, Published: APR 2018 Revista da Sociedade Brasileira de Medicina Tropical v.51 n.2 2018 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 2, Pp 190-197 |
ISSN: | 1678-9849 |
Popis: | INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key. The analysis of natural infection by T. cruzi in the insects was performed by isolation in culture and polymerase chain reaction. The antibody testing for T. cruzi in dogs was performed by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. RESULTS: A total of 68 triatomines were captured, predominantly the Triatoma brasiliensis brasiliensis (Neiva 1911) species. The vector mapping displayed areas with greater risk for parasite transmission. Of the examined triatomines (51 specimens), 41.2% (21/51) were positive on polymerase chain reaction, and all were negative on culture. In the serum testing, 11% (25/218) of dogs were seropositive, but no association was found between the serologic results and the presence and infection by T. cruzi in triatomines. CONCLUSIONS: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission. |
Databáze: | OpenAIRE |
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