Plant Roots Release Small Extracellular Vesicles with Antifungal Activity
| Autor: | Ramesh Bokka, Lilla Turiák, Antonietta Leone, Pasquale Del Gaudio, Alfredo Ambrosone, Immacolata Fiume, Gabriella Pocsfalvi, Marina Tucci, Monica De Palma, Michelina Ruocco |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Botrytis root exudate Plant Science tomato 01 natural sciences Alternaria alternata extracellular vesicles plant-pathogen interactions proteomics fungal pathogens biocargo Fusarium Alternaria Article 03 medical and health sciences food Fusarium oxysporum Spore germination Plant defense against herbivory Biocargo Extracellular vesicles Fungal pathogens Plant-pathogen interactions Proteomics Root exudate Tomato Ecology Evolution Behavior and Systematics Botrytis cinerea Innate immune system Ecology biology Chemistry Vesicle fungi Botany food and beverages biology.organism_classification Cell biology 030104 developmental biology QK1-989 010606 plant biology & botany |
| Zdroj: | Plants Plants 9 (2020). doi:10.3390/plants9121777 info:cnr-pdr/source/autori:De Palma M., Ambrosone A., Leone A., Del Gaudio P., Ruocco M., Turiák L., Bokka R., Fiume I., Tucci M., Pocsfalvi G./titolo:Plant Roots Release Small Extracellular Vesicles with Antifungal Activity/doi:10.3390%2Fplants9121777/rivista:Plants/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:9 Volume 9 Issue 12 Plants, Vol 9, Iss 1777, p 1777 (2020) |
| ISSN: | 2223-7747 |
| Popis: | Extracellular Vesicles (EVs) play pivotal roles in cell-to-cell and inter-kingdom communication. Despite their relevant biological implications, the existence and role of plant EVs released into the environment has been unexplored. Herein, we purified round-shaped small vesicles (EVs) by differential ultracentrifugation of a sampling solution containing root exudates of hydroponically grown tomato plants. Biophysical analyses, by means of dynamic light scattering, microfluidic resistive pulse sensing and scanning electron microscopy, showed that the size of root-released EVs range in the nanometric scale (50&ndash 100 nm). Shot-gun proteomics of tomato EVs identified 179 unique proteins, several of which are known to be involved in plant-microbe interactions. In addition, the application of root-released EVs induced a significant inhibition of spore germination and of germination tube development of the plant pathogens Fusarium oxysporum, Botrytis cinerea and Alternaria alternata. Interestingly, these EVs contain several proteins involved in plant defense, suggesting that they could be new components of the plant innate immune system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|