Genome-wide screen reveals important roles for ESCRT proteins in drug/ion resistance of fission yeast
| Autor: | Si Chen, Fan Yao, Guanglie Jiang, Lina Zhou, Norihiro Sakamoto, Qiannan Liu, Yikun Yang, Takayoshi Kuno, Yue Fang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Mutagenesis and Gene Deletion Techniques lcsh:Medicine Amiodarone Yeast and Fungal Models Vacuole Sodium Chloride Biochemistry Schizosaccharomyces Pombe Medicine and Health Sciences Homeostasis lcsh:Science Multidisciplinary biology Chemistry Calcineurin Fungal genetics Eukaryota Salt Tolerance Cell biology Experimental Organism Systems Physical Sciences Saccharomyces Cerevisiae Genome Fungal Cellular Structures and Organelles Schizosaccharomyces Research Article inorganic chemicals Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Vacuole fusion macromolecular substances Research and Analysis Methods Microbiology ESCRT 03 medical and health sciences Saccharomyces Model Organisms Drug Resistance Fungal Microbial Control DNA-binding proteins Molecular Biology Techniques Molecular Biology Pharmacology 030102 biochemistry & molecular biology Endosomal Sorting Complexes Required for Transport lcsh:R Deletion Mutagenesis Organisms Fungi Chemical Compounds Biology and Life Sciences Proteins Cell Biology biology.organism_classification Yeast Tamoxifen 030104 developmental biology Ion homeostasis Schizosaccharomyces pombe Mutation Vacuoles lcsh:Q Salts Antimicrobial Resistance Physiological Processes |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 6, p e0198516 (2018) |
| ISSN: | 1932-6203 |
| Popis: | To study sodium homeostasis, we performed a genome-wide screen for deletion strains that show resistance to NaCl. We identified 34 NaCl-resistant strains. Among them, the largest group that consists of 10 genes related to membrane trafficking and 7 out of 10 genes are ESCRT proteins which are involved in cargo transportation into luminal vesicles within the multivesicular body. All of the ESCRT related mutants which showed sodium resistance also showed defects in vacuole fusion. To further understand the role of the ESCRT pathway in various ion homeostasis, we examined sensitivity of these ESCRT mutants to various cation salts other than NaCl, including KCl, LiCl, CaCl2, CoCl2, MgCl2, NiSO4 and MnCl2. While these ESCRT mutants showed resistance to LiCl, CoCl2 and MgCl2, they showed sensitivity to KCl, CaCl2, NiSO4 and MnCl2. Then we examined sensitivity of these ESCRT mutants to various drugs which are known to inhibit the growth of fission yeast cells. While these ESCRT mutants were more or equally sensitive to most of the drugs tested as compared to the wild-type cells, they showed resistance to some drugs such as tamoxifen, fluorouracil and amiodarone. These results suggest that the ESCRT pathway plays important roles in drug/ion resistance of fission yeast. |
| Databáze: | OpenAIRE |
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