Evaluation of Toxicity and Neural Uptake In Vitro and In Vivo of Superparamagnetic Iron Oxide Nanoparticles

Autor: Berend van Wachem, Alexander Dityatev, Petra Henrich-Noack, Lisa Grigartzik, Enqi Zhang, Muhammad Kamran Khalid, Bernhard A. Sabel, Werner Hintz, Maxim Sokolov, Muhammad Usman Asad
Jazyk: angličtina
Rok vydání: 2018
Předmět:
02 engineering and technology
superparamagnetic iron oxide nanoparticles (SPIO-NPs)
Ferric Compounds
01 natural sciences
Rhodamine 123
lcsh:Chemistry
chemistry.chemical_compound
Magnetite Nanoparticles
Cytotoxicity
lcsh:QH301-705.5
Spectroscopy
Chemistry
Glioma
General Medicine
021001 nanoscience & nanotechnology
Computer Science Applications
Dextran
ferric oxide
ddc:540
Drug delivery
0210 nano-technology
chemistry [Magnetite Nanoparticles]
Cell Survival
drug effects [Cell Survival]
Confocal
0699 Other Biological Sciences
in vitro toxicity
010402 general chemistry
Article
Catalysis
Inorganic Chemistry
In vivo
Cell Line
Tumor
0399 Other Chemical Sciences
toxicity [Magnetite Nanoparticles]
Animals
Physical and Theoretical Chemistry
Molecular Biology
0604 Genetics
Chemical Physics
Organic Chemistry
cellular uptake
In vitro
Rats
0104 chemical sciences
chemistry [Ferric Compounds]
lcsh:Biology (General)
lcsh:QD1-999
drug delivery
Biophysics
Ex vivo
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 9
International Journal of Molecular Sciences, Vol 19, Iss 9, p 2613 (2018)
International journal of molecular sciences 19(9), 2613 (2018). doi:10.3390/ijms19092613
ISSN: 1422-0067
DOI: 10.3390/ijms19092613
Popis: Superparamagnetic iron oxide nanoparticles (SPIO-NPs) have great potential to be used in different pharmaceutical applications, due to their unique and versatile physical and chemical properties. The aim of this study was to quantify in vitro cytotoxicity of dextran 70,000-coated SPIO-NPs labelled/unlabelled with rhodamine 123, in C6 glioma cells and primary hippocampal neural cells. In addition, we analyzed the in vitro and in vivo cellular uptake of labelled SPIO-NPs. The nanoparticles, with average size of 10&ndash
50 nm and polydispersity index of 0.37, were synthesized using Massart&rsquo
s co-precipitation method. The concentration-dependent cytotoxicity was quantified by using tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Intracellular localization of SPIO-NPs was detected by confocal laser microscopy. In vivo confocal neuroimaging (ICON) was performed on male Wistar rats after intravitreal injection followed by ex vivo retina whole mount analysis. When used for in vitro testing concentrations in the range of diagnostic and therapeutic dosages, SPIO-NPs proved to be non-cytotoxic on C6 glioma cells for up to 24 h incubation time. The hippocampal cell culture also did not show impaired viability at low doses after 24 h incubation. Our results indicate that our dextran-coated SPIO-NPs have the potential for in vivo drug delivery applications.
Databáze: OpenAIRE
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