Preparation and characterization of poly(l-phenylalanine) chiral stationary phases with varying peptide length
| Autor: | Kaname Ohyama, Kenichiro Nakashima, Mitsuhiro Wada, Yoshihito Ohba, Naotaka Kuroda, Toshihide Maki, Naoya Kishikawa, Kana Oyamada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Stereochemistry
Phenylalanine Liquid chromatography Infrared spectroscopy Peptide Biochemistry Peptide length Analytical Chemistry chemistry.chemical_compound Chiral stationary phases Secondary structure Peptide synthesis Protein secondary structure Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Organic Chemistry l-Phenylalanine Stereoisomerism General Medicine Silicon Dioxide Polymerization chemistry Reagent Warfarin Enantiomer Peptides |
| Zdroj: | Journal of chromatography. A. 1208(1-2):242-245 |
| ISSN: | 0021-9673 |
| Popis: | Three new chiral stationary phases with different lengths of l-phenylalanine peptide were prepared by solid-phase synthesis with tert-butoxycarbonyl (Boc)-l-phenylalanine on silica. The effect of phenylalanine peptide length on enantioselectivity was studied. The best separation of R/S-warfarin was achieved by the chiral stationary phase with intermediate peptide length. These stationary phases were found to exist mainly in alpha-helical conformation by using FT-IR spectra. The end-capping reagents for the N-terminus of the peptide were also evaluated. Journal of Chromatography A, 1208(1-2), pp.242-245; 2008 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect