Microfluidic device for single-cell analysis
| Autor: | Antoine Daridon, Rebecca J. Whelan, Ian David Manger, Andrew M. Leach, Aaron R. Wheeler, Richard N. Zare, William Throndset, Kevin Farrell, Yish Hann Liao |
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| Rok vydání: | 2003 |
| Předmět: |
Chemistry
Photochemistry Cells Microfluidics Silicones Nanotechnology U937 Cells Suspension culture Analytical Chemistry Jurkat Cells Single-cell analysis Multilayer soft lithography Microcomputers Microscopy Fluorescence Temporal resolution Reagent Humans Calcium Indicators and Reagents Dimethylpolysiloxanes Lithography Intracellular Cells Cultured Fluorescent Dyes |
| Zdroj: | Analytical chemistry. 75(14) |
| ISSN: | 0003-2700 |
| Popis: | We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated, including cell viability assays, ionophore-mediated intracellular Ca2+ flux measurements, and multistep receptor-mediated Ca2+ measurements. These assays, and others, are achieved with significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives. |
| Databáze: | OpenAIRE |
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